Featured products at Synvolux Therapeutics:

    - Fully synthetic transfection reagent
    - Serum transfection compatible
    - Efficiencies up 96%
    - Free of animal-derived components

    - Fully synthetic transfection reagent
    - Only 4pmol siRNA needed
    - Long-term siRNA storage
    - Free of animal-derived components

    - Fully synthetic transfection reagent
    - Functionality preserved
    - Up to 98% efficiency
    - Free of animal-derived components

SAINT-O-Somes for improved intracellular delivery

In non-phagocytic cells such as endothelial cells, processing of liposomes and subsequent release of drug content is often slow which limits pharmacological efficacy. We developed a liposome based drug delivery system with superior intracellular release characteristics in cells that lack an efficient processing machinery. Long circulating liposomes were formulated with different molar ratios of the cationic amphyphile 1-methyl-4-(cis-9-dioleyl)methyl-pyridinium-chlorid (SAINT-C18). These SAINT-O-Somes were as stable as conventionally formulated liposomes, but showed superior content release characteristics at pH conditions that liposomes encounter when they are endocytosed by cells. Attachment of anti-E-selectin specific antibodies to the distal end of surface grafted poly(ethylene glycol) resulted in immuno-SAINT-O-Somes that were as efficiently taken up by tumor necrosis-a activated endothelial cells as conventional anti-E-selectin specific immunoliposomes. More importantly, intracellular release of calcein encapsulated in these targeted SAINT-O-Somes was 10 fold higher as compared to the release of calcein from conventional liposomes. For intracellular delivery siRNA into activated endothelial cells, formulation with SAINT-C18 was a necessity, as demonstrated by the specific down-regulation of expression of VE-cadherin. SAINT-O-Somes therefore represent a new class of lipid based particles with superior drug release characteristics that can be applied for the efficacious intracellular delivery of hydrophilic drugs including siRNA.

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Keywords: liposomes, Saint, endothelial cells, intracellular release, siRNA

Transfection is the process of introducing nucleic acids into cells by non-viral methods.

DNA, siRNA or protein transfection reagent allow the uptake of material. Genetic material (such as supercoiled plasmid DNA or plasmid fragments), siRNA or even proteins such as antibodies, may be transfected. In addition to transfection performed with lipoplexes, transfection can be carried out using the classical calcium phosphate transfection, electroporation devices, or by mixing a cationic lipid with the material to produce liposomes and or Saint o somes. These three classical methods induce fusion with the cell plasma membrane and endocytosis which hopefully results in the deposition of their cargo inside the cell.

Synvolux developed a new series of transfection reagents and transfection protocols which guarantee a high cell transfection efficacy combined with minimal cell death. Also very capable for high volume siRNA throughput screening with low amounts siRNA.

For plasmid DNA transfection we recommend SAINT-MIX.

Transfection of primary cells with high transfection efficacy is achieved with SAINT-18.

Normally transient transfection is achieved, however when applying a selection marker stable transfection efficacy will be high and you will easily retrieve stable cell lines expressing the protein of interest.

SiRNA transfection with minimal amounts of siRNA and maximal down-regulation of the targeted gene is achieved with SAINT-RED.

Protein transfection protcols can be found in the SAINT-PhD (Protein high Delivery) kit.

For therapeutical applications we develop the SAINT-O-SOMES methods which can be prepared by using SAINT-Solid-1.

The term transformation is preferred to describe non-viral DNA transfer in bacteria. Our product TRAEN, TRAnsformation ENhancer, will boost your bacterial colonies retrieved after transformation by 10 times. The protocol is very friendly and extremely simple.